LUPUS ANTICOAGULANT EVALUATION WITH REFLEX

SPECIMEN: 3 ML FROZEN PLATELET POOR PLASMA

TUBE TYPE: 3.2% SODIUM CITRATE (LIGHT BLUE-TOP) TUBE

MINIMUM VOLUME: 2 ML

TRANSPORT: FROZEN; PLATELET-POOR PLASMA: CENTRIFUGE LIGHT BLUE-TOP TUBE FOR 15 MINUTES AT APPROXIMATELY 1500 G WITHIN 60 MINUTES OF COLLECTION. USING A PLASTIC PIPETTE, REMOVE PLASMA, TAKING CARE TO AVOID THE WBC/PLATELET BUFFY LAYER AND PLACE INTO A PLASTIC VIAL. CENTRIFUGE A SECOND TIME AND TRANSFER PLATELET-POOR PLASMA INTO A NEW PLASTIC VIAL. PLASMA MUST BE FREE OF PLATELETS(<10,000/μL). FREEZE IMMEDIATELY