PROTEIN C ANTIGEN

SPECIMEN: 1 ML FROZEN PLASMA

TUBE TYPE 3.2% SODIUM CITRATE (LIGHT BLUE TOP) TUBE

MINIMUM VOLUME: 0.5ML

TRANSPORT: FROZEN; DRAW BLOOD IN LIGHT BLUE-TOP TUBE CONTAINING 3.2% SODIUM CITRATE. MIX GENTLY BY INVERTING 3-4 TIMES. CENTRIFUGE 15 MINUTES AT 1500 G WITHIN 1 HOUR OF COLLECTION. USING A PLASTIC PIPETTE, REMOVE PLASMA, TAKING CARE TO AVOID THE WBC/PLATELET (BUFFY) LAYER AND PLACE INTO A PLASTIC VIAL. CENTRIFUGE A SECOND TIME AND TRANSFER PLATELET-POOR PLASMA INTO A NEW PLASTIC VIAL. FREEZE IMMEDIATELY AND SHIP.